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Corning Life Sciences transwell clear polyester membrane inserts
Transwell Clear Polyester Membrane Inserts, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyester+membrane+transwell-clear+inserts/96+well+plates/pm40435350-43-13-11
Average 90 stars, based on 1 article reviews
transwell clear polyester membrane inserts - by Bioz Stars, 2026-11
90/100 stars

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Membrane:

Article Title: miR-200b inhibits TNF-α-induced IL-8 secretion and tight junction disruption of intestinal epithelial cells in vitro.
Article Snippet: .. For establishing the monolayer, the Caco-2 cells were, respectively, seeded on polyester membrane Transwell-clear inserts with a 0.33-, 1.12-, or 4.67-cm2 growth area (6.50-mm diameter, 0.4-mm pore size; Corning, Corning, NY) at 5 104, 1.5 105, and 5 105 cells/well and grown for 10–14 days postconfluence. .. The cells on a 0.33-, 1.12-, and 4.67-cm2 Transwell insert (cat. no. 3470, 3460, and 3450; Corning) were used for assessment of transepithelial electrical resistance (TEER) and paracellular permeability, immunofluorescence assa,y and Western blot assay, respectively.

Article Title: Inhibition of IL-13-induced periostin in airway epithelium attenuates cellular protein expression of MUC5AC.
Article Snippet: Background and objective: Serum periostin is increased in asthma and serves as a surrogate marker for IL-13 activity in the lung.. Serum levels of periostin are the most robust biomarker predicting a favourable response to the anti-IL-13 drug, lebrikizumab.. We investigated the mechanisms of IL-13 stimulation of periostin, the polarized secretion of periostin and whether periostin would have a direct effect on mucin secretion by airway cells.

Article Title: Protein Kinase Cζ Inhibitor Promotes Resolution of Bleomycin-Induced Acute Lung Injury
Article Snippet: Dead cells were eliminated with Hoechst #33258 positive staining (Invitrogen). .. Mouse integrin β4+ cell proliferation assay Isolated integrin β4+ cell suspension (5x104 in 50 μl) in Small Airway Basal Medium (Clonetics) were mixed 1:1 at 4°C with matrigel and seeded on top of 6.5 mm polyester membrane transwell-clear inserts (Cat. No. 3470, Corning). ..

Article Title: Mesenchymal stem cells may alleviate angiotensin II-induced myocardial fibrosis and hypertrophy by upregulating SFRS3 expression.
Article Snippet: Cell co-culture To assess the impact of rMSCs on rCFBs or rCMCs, rCFBs or rCMCs were seeded at a density of 2 × 104 cells/cm2 in 6-well plates and cultured with 0.1 μM Ang II (#A9290; Solarbio, China). .. Simultaneously, rMSCs (4 × 104 cells/cm2) were seeded onto polyester membrane Transwell-clear inserts (pore size 0.4 μm; Corning, USA), which were placed into each well of the 6-well plates for coculturing with rCFBs or rCMCs. ..

Pore Size:

Article Title: miR-200b inhibits TNF-α-induced IL-8 secretion and tight junction disruption of intestinal epithelial cells in vitro.
Article Snippet: .. For establishing the monolayer, the Caco-2 cells were, respectively, seeded on polyester membrane Transwell-clear inserts with a 0.33-, 1.12-, or 4.67-cm2 growth area (6.50-mm diameter, 0.4-mm pore size; Corning, Corning, NY) at 5 104, 1.5 105, and 5 105 cells/well and grown for 10–14 days postconfluence. .. The cells on a 0.33-, 1.12-, and 4.67-cm2 Transwell insert (cat. no. 3470, 3460, and 3450; Corning) were used for assessment of transepithelial electrical resistance (TEER) and paracellular permeability, immunofluorescence assa,y and Western blot assay, respectively.

Article Title: Mesenchymal stem cells may alleviate angiotensin II-induced myocardial fibrosis and hypertrophy by upregulating SFRS3 expression.
Article Snippet: Cell co-culture To assess the impact of rMSCs on rCFBs or rCMCs, rCFBs or rCMCs were seeded at a density of 2 × 104 cells/cm2 in 6-well plates and cultured with 0.1 μM Ang II (#A9290; Solarbio, China). .. Simultaneously, rMSCs (4 × 104 cells/cm2) were seeded onto polyester membrane Transwell-clear inserts (pore size 0.4 μm; Corning, USA), which were placed into each well of the 6-well plates for coculturing with rCFBs or rCMCs. ..

other:

Article Title: IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma
Article Snippet: Migration assays were performed using the Polyester Membrane Transwell-Clear Inserts (cat. 3472; Corning, Tewksbury, MA).

Article Title: Delivery of BMP-2 by two clinically available apatite materials: in vitro and in vivo comparison.
Article Snippet: MC3T3E1 cells were seeded in 1 mL cell culture medium on the bottom of 24-well plates in differentiation medium and a transwell insert added to the well (Polyester Membrane Transwell-Clear Inserts, 24-well plate, 3.0 mm pore size, Corning, NY). rhBMP-2-loaded granules and control granules were placed in the transwell inserts shown to be permeable for rhBMP-2. rhBMP-2 for positive controls was added to the upper part of the transwell compartment and cells were incubated at 37 C, 5% CO2.

Article Title: Tubastatin A Prevents Hemorrhage-Induced Endothelial Barrier Dysfunction
Article Snippet: Monolayer Permeability Assay HUVECs were seeded onto Polyester Membrane Transwell-Clear Inserts (0.4 μm pore, 6.5 mm diameter; Corning Inc., Corning, NY), and exposed to the treatment once they were at 100% confluency (3 days after seeding) for 30 hours.

Article Title: Amniotic Fluid Stem Cells Rescue Both in Vitro and in Vivo Growth, Innervation, and Motility in Nitrofen-Exposed Hypoplastic Rat Lungs through Paracrine Effects
Article Snippet: Received November 16, 2011; final acceptance August 27, 2012.. Online prepub date: October 8, 2012.. These authors provided equal contribution to this work.

Proliferation Assay:

Article Title: Protein Kinase Cζ Inhibitor Promotes Resolution of Bleomycin-Induced Acute Lung Injury
Article Snippet: Dead cells were eliminated with Hoechst #33258 positive staining (Invitrogen). .. Mouse integrin β4+ cell proliferation assay Isolated integrin β4+ cell suspension (5x104 in 50 μl) in Small Airway Basal Medium (Clonetics) were mixed 1:1 at 4°C with matrigel and seeded on top of 6.5 mm polyester membrane transwell-clear inserts (Cat. No. 3470, Corning). ..

Isolation:

Article Title: Protein Kinase Cζ Inhibitor Promotes Resolution of Bleomycin-Induced Acute Lung Injury
Article Snippet: Dead cells were eliminated with Hoechst #33258 positive staining (Invitrogen). .. Mouse integrin β4+ cell proliferation assay Isolated integrin β4+ cell suspension (5x104 in 50 μl) in Small Airway Basal Medium (Clonetics) were mixed 1:1 at 4°C with matrigel and seeded on top of 6.5 mm polyester membrane transwell-clear inserts (Cat. No. 3470, Corning). ..

Suspension:

Article Title: Protein Kinase Cζ Inhibitor Promotes Resolution of Bleomycin-Induced Acute Lung Injury
Article Snippet: Dead cells were eliminated with Hoechst #33258 positive staining (Invitrogen). .. Mouse integrin β4+ cell proliferation assay Isolated integrin β4+ cell suspension (5x104 in 50 μl) in Small Airway Basal Medium (Clonetics) were mixed 1:1 at 4°C with matrigel and seeded on top of 6.5 mm polyester membrane transwell-clear inserts (Cat. No. 3470, Corning). ..



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Impact of miR-enriched-sEVs in the static human in vitro BBB model. a Schematic representation of the experimental setup. The BBB phenotype was established through 4 days of co-culture in <t>Transwell</t> systems. The modulated sEVs (10 10 sEVs/mL) were incubated for 48 h with the BECs, then the medium was replaced with fresh medium and the ECs monolayer integrity was evaluated through TEER, paracellular permeability and cell viability. b Basal expression of the miRNAs used for the sEVs modulation in the BECs. Values are mean ± SEM (n = 3 independent experiment with 3 technical replicates). c Paracellular permeability (Pe) to lucifer yellow (LY), d TEER values and e BEC viability after 48 h of incubation with miR-enriched sEVs. Cell viability was assessed by a PrestoBlue assay. The values are normalized to the respective controls (miR-scr-sEVs) and expressed as mean ± SEM, n = 2–4 independent experiments with 3 technical replicates (each experiment with a different batch of sEVs, therefore 3 different donors). Unpaired t test between each condition and the BBB model incubated with the miR-scr-sEVs was performed as statistical analysis. *, **, and *** denote statistical significance (p < 0.05, p < 0.01, p < 0.001). f Gene expression analysis of claudin 5, ZO-1, vWF, and occludin in BECs after 48 h incubation with 10 10 miR-383-3p-sEVs/mL. The results are mean ± SEM, n = 4 independent experiments with 3 technical replicates. g Representative confocal images of claudin 5, ZO-1, β-catenin, occludin, and vWF after the incubation with the miR-scr-sEVs and with miR-383-3p-sEVs. Scale bar is 20 μm. h Mean intensity of the immunostaining for claudin 5, ZO-1, β-catenin, occludin, and vWF after incubation with miR-enriched sEVs. The values were normalized by miR-scr-sEVs condition. The data are mean ± SEM, n = 3–6 independent experiments with 2–4 images per experiment. Statistical analysis was performed with an unpaired student’s t -test, *** p < 0.001
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Impact of miR-enriched-sEVs in the static human in vitro BBB model. a Schematic representation of the experimental setup. The BBB phenotype was established through 4 days of co-culture in <t>Transwell</t> systems. The modulated sEVs (10 10 sEVs/mL) were incubated for 48 h with the BECs, then the medium was replaced with fresh medium and the ECs monolayer integrity was evaluated through TEER, paracellular permeability and cell viability. b Basal expression of the miRNAs used for the sEVs modulation in the BECs. Values are mean ± SEM (n = 3 independent experiment with 3 technical replicates). c Paracellular permeability (Pe) to lucifer yellow (LY), d TEER values and e BEC viability after 48 h of incubation with miR-enriched sEVs. Cell viability was assessed by a PrestoBlue assay. The values are normalized to the respective controls (miR-scr-sEVs) and expressed as mean ± SEM, n = 2–4 independent experiments with 3 technical replicates (each experiment with a different batch of sEVs, therefore 3 different donors). Unpaired t test between each condition and the BBB model incubated with the miR-scr-sEVs was performed as statistical analysis. *, **, and *** denote statistical significance (p < 0.05, p < 0.01, p < 0.001). f Gene expression analysis of claudin 5, ZO-1, vWF, and occludin in BECs after 48 h incubation with 10 10 miR-383-3p-sEVs/mL. The results are mean ± SEM, n = 4 independent experiments with 3 technical replicates. g Representative confocal images of claudin 5, ZO-1, β-catenin, occludin, and vWF after the incubation with the miR-scr-sEVs and with miR-383-3p-sEVs. Scale bar is 20 μm. h Mean intensity of the immunostaining for claudin 5, ZO-1, β-catenin, occludin, and vWF after incubation with miR-enriched sEVs. The values were normalized by miR-scr-sEVs condition. The data are mean ± SEM, n = 3–6 independent experiments with 2–4 images per experiment. Statistical analysis was performed with an unpaired student’s t -test, *** p < 0.001
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Image Search Results


Journal: iScience

Article Title: Pathogenic KIAA0586/TALPID3 variants are associated with defects in primary and motile cilia

doi: 10.1016/j.isci.2024.111670

Figure Lengend Snippet:

Article Snippet: Costar Corning Transwell clear polyester membrane inserts , Corning Inc. , Cat#3470.

Techniques: Control, Recombinant, Fluorescence, Sequencing, Isolation, Software, Membrane, Microscopy

Impact of miR-enriched-sEVs in the static human in vitro BBB model. a Schematic representation of the experimental setup. The BBB phenotype was established through 4 days of co-culture in Transwell systems. The modulated sEVs (10 10 sEVs/mL) were incubated for 48 h with the BECs, then the medium was replaced with fresh medium and the ECs monolayer integrity was evaluated through TEER, paracellular permeability and cell viability. b Basal expression of the miRNAs used for the sEVs modulation in the BECs. Values are mean ± SEM (n = 3 independent experiment with 3 technical replicates). c Paracellular permeability (Pe) to lucifer yellow (LY), d TEER values and e BEC viability after 48 h of incubation with miR-enriched sEVs. Cell viability was assessed by a PrestoBlue assay. The values are normalized to the respective controls (miR-scr-sEVs) and expressed as mean ± SEM, n = 2–4 independent experiments with 3 technical replicates (each experiment with a different batch of sEVs, therefore 3 different donors). Unpaired t test between each condition and the BBB model incubated with the miR-scr-sEVs was performed as statistical analysis. *, **, and *** denote statistical significance (p < 0.05, p < 0.01, p < 0.001). f Gene expression analysis of claudin 5, ZO-1, vWF, and occludin in BECs after 48 h incubation with 10 10 miR-383-3p-sEVs/mL. The results are mean ± SEM, n = 4 independent experiments with 3 technical replicates. g Representative confocal images of claudin 5, ZO-1, β-catenin, occludin, and vWF after the incubation with the miR-scr-sEVs and with miR-383-3p-sEVs. Scale bar is 20 μm. h Mean intensity of the immunostaining for claudin 5, ZO-1, β-catenin, occludin, and vWF after incubation with miR-enriched sEVs. The values were normalized by miR-scr-sEVs condition. The data are mean ± SEM, n = 3–6 independent experiments with 2–4 images per experiment. Statistical analysis was performed with an unpaired student’s t -test, *** p < 0.001

Journal: Journal of Nanobiotechnology

Article Title: Engineering extracellular vesicles to transiently permeabilize the blood–brain barrier

doi: 10.1186/s12951-024-03019-w

Figure Lengend Snippet: Impact of miR-enriched-sEVs in the static human in vitro BBB model. a Schematic representation of the experimental setup. The BBB phenotype was established through 4 days of co-culture in Transwell systems. The modulated sEVs (10 10 sEVs/mL) were incubated for 48 h with the BECs, then the medium was replaced with fresh medium and the ECs monolayer integrity was evaluated through TEER, paracellular permeability and cell viability. b Basal expression of the miRNAs used for the sEVs modulation in the BECs. Values are mean ± SEM (n = 3 independent experiment with 3 technical replicates). c Paracellular permeability (Pe) to lucifer yellow (LY), d TEER values and e BEC viability after 48 h of incubation with miR-enriched sEVs. Cell viability was assessed by a PrestoBlue assay. The values are normalized to the respective controls (miR-scr-sEVs) and expressed as mean ± SEM, n = 2–4 independent experiments with 3 technical replicates (each experiment with a different batch of sEVs, therefore 3 different donors). Unpaired t test between each condition and the BBB model incubated with the miR-scr-sEVs was performed as statistical analysis. *, **, and *** denote statistical significance (p < 0.05, p < 0.01, p < 0.001). f Gene expression analysis of claudin 5, ZO-1, vWF, and occludin in BECs after 48 h incubation with 10 10 miR-383-3p-sEVs/mL. The results are mean ± SEM, n = 4 independent experiments with 3 technical replicates. g Representative confocal images of claudin 5, ZO-1, β-catenin, occludin, and vWF after the incubation with the miR-scr-sEVs and with miR-383-3p-sEVs. Scale bar is 20 μm. h Mean intensity of the immunostaining for claudin 5, ZO-1, β-catenin, occludin, and vWF after incubation with miR-enriched sEVs. The values were normalized by miR-scr-sEVs condition. The data are mean ± SEM, n = 3–6 independent experiments with 2–4 images per experiment. Statistical analysis was performed with an unpaired student’s t -test, *** p < 0.001

Article Snippet: Six hours after, ECs were seeded (80,000 cells/insert) in Transwell ® -Clear Inserts with Polyester (PET) membrane (Corning, 3460) or Transwell ® permeable supports with polycarbonate membrane (Corning, 3401) previously coated with MatrigelTM (Basement Membrane Matrix, 10 mL *LDEV-Free, BD) diluted 1:48 in cold Dulbecco’s Modified Eagle’s Medium (DMEM) for 1 h at room temperature (RT).

Techniques: In Vitro, Co-Culture Assay, Incubation, Permeability, Expressing, Prestoblue Assay, Gene Expression, Immunostaining